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KMID : 0385520080210040245
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Analytical Science & Technology
2008 Volume.21 No. 4 p.245 ~ p.248
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Structural determination of triterpenic acids in Prunellae Spica by fast atom bombardment tandem mass spectrometry
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Anh Young-Min
Hong Jong-Kee
Lee Kang-Ro
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Abstract
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Five triterpenic acids as marker compounds were extracted and isolated from Prunellae Spica by column chromatography and reversed-phase high-performance liquid chromatography (HPLC), and their purity was determinated by HPLC (purity ≥ 90%). Molecular weight and elemental compositions of the five marker compounds were determined by fast atom bombardment high-resolution mass spectrometry (FAB-HRMS). The structural determination of the five marker compounds was carried out fast atom bombardment collision-induced dissociation tandem mass spectrometry (FAB-CID-MS/MS). The collision-induced dissociation (CID) of protonated molecules [M+H]? and deprotonated molecules [M-H]? produced diverse product ions due mainly to retro Diels-Alder reaction (RDA), dehydration and decarboxylation. Moreover, the CID-MS/MS spectra of the [M-H]? ions were observed charge-remote fragmentation (CRF) patterns. On the basis of interpretation of CID-MS/MS spectra, structural elucidation of triterpenic acids isolated from Prunellae Spica was clearly performed.
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KEYWORD
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Prunellae Spica, triterpenic acid, structural analysis, FAB-CID-MS/MS, dissociation patterns
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